Destas, 64% foram positivas tambГ©m na reaГ§ГЈo em cadeia da polimerase. Das 169 negativas, 8% foram positivas na imunofluorescГЄncia indireta. Destas quatro discordantes, 25% apresentaram vГrus da dengue nГЈo detectado na reaГ§ГЈo em cadeia da polimerase. Das 31 positivas, 12,9% foram negativas na imunofluorescГЄncia indireta. Para IgG antivГrus da dengue, de 200 amostras, 15,5% foram positivas e 84,5% negativas no ensaio imunoenzimГЎtico. Sensibilidade e especificidade por imunofluorescГЄncia indireta foram, respectivamente, 75% e 94%. Por fim, dentre as nove discordantes, 33% tiveram vГrus da dengue detectado na reaГ§ГЈo em cadeia da polimerase. Das 160 negativas no ensaio imunoenzimГЎtico, 5% foram positivas na imunofluorescГЄncia indireta. Destas dez negativas, apenas 20% eram tambГ©m negativas na reaГ§ГЈo em cadeia da polimerase. Das 40 positivas, 25% foram negativas na imunofluorescГЄncia indireta. Resultados:Дas 200 amostras, 20% foram positivas e 80% negativas para IgM antivГrus da dengue no ensaio imunoenzimГЎtico. Nas amostras discordantes, realizou-se reaГ§ГЈo em cadeia da polimerase como mГ©todo confirmatГіrio. Foram calculados especificidade, sensibilidade e coeficiente Kappa. MГ©todos:Дuzentas amostras de soro de pacientes com suspeita clГnica de dengue foram testadas por ensaio imunoenzimГЎtico e imunofluorescГЄncia indireta mosaico BIOCHIP В®. However, indirect immunofluorescence can distinguish three arboviruses simultaneously, an advantage during concomitant epidemics.ДЄrbovirus infections Dengue Enzyme-linked immunosorbent assay Serologic tests Fluorescent antibody technique indirectДЄvaliar o desempenho da imunofluorescГЄncia indireta no diagnГіstico sorolГіgico de dengue em uma populaГ§ГЈo com alta prevalГЄncia de arboviroses. Replacing the immunoenzymatic test by indirect immunofluorescence would compromise the sensitivity for IgM. Conclusion:ДЇor diagnosis of acute infection, the immunoenzymatic test is enough, and the use of additional methods is not warranted. Sensitivity and specificity of indirect immunofluorescence were 87.1% and 91.7%, respectively. Of these 14 discordant results, 64% were also positive in the PCR. Of the 169 negatives, 8% were positive in indirect immunofluorescence. Of these four discordant results, 25% were negative in the PCR. Of the 31 positives, 12.9% were negative in indirect immunofluorescence. For anti-dengue virus IgG antibodies, of the 200 samples, 15.5% were positive and 84.5% were negative in the immunoenzymatic test. Sensitivity and specificity of indirect immunofluorescence were respectively 75% and 94%. Of these nine discordant results, 33% were positive in the PCR. Of the 160 negatives in the immunoenzymatic test, 5% were positive in indirect immunofluorescence. Of these ten discordant results, only 20% were also negative in the polymerase chain reaction (PCR). Of the 40 positives, 25% were negative in indirect immunofluorescence. Of the 200 samples, 20% were positive and 80% negative for anti-dengue virus IgM antibodies in the immunoenzymatic test. Discordant samples were tested by polymerase chain reaction for confirmation. Specificity, sensitivity and Kappa coefficient were calculated. Two-hundred serum samples from patients with clinical suspicion of dengue fever were tested by immunoenzymatic and indirect immunofluorescence assay BIOCHIPВ® mosaic. To evaluate the performance of indirect immunofluorescence for serological diagnosis of dengue virus in a population with high prevalence of arboviruses.

Ep evaluator cost